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DSMZ

DSMZ 906 . DETHIOSULFOVIBRIO MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 906 #

ml
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 19.98 341.889
MgCl2 x 6 H2O 3.0 g 2.997 14.742
KH2PO4 1.0 g 0.999 7.341
Na3-citrate x 5 H2O 3.0 g 2.997 -
Yeast extract
(OXOID) 		5.0 g 4.995 -
Peptone
(BD Bacto) 		2.0 g 1.998 -
Trace element solution SL-10 1.0 ml - -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
CaCl2 x 2 H2O 0.2 g 0.2 1.359
Na2S2O3 x 5 H2O 2.5 g 2.498 10.063
Na2S x 9 H2O 0.5 g 0.5 2.08
Distilled water 1000.0 ml - -
1 Dissolve ingredients except calcium chloride, thiosulfate and sulfide. Sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The pH of the complete medium should be at 6.7 - 6.8.

Trace element solution SL-10 #

1000
Solution occurs in 265 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 08.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.7 - 6.8
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.98 341.889
Yeast extract 4.995 -
MgCl2 x 6 H2O 2.997 14.742
Na3-citrate x 5 H2O 2.997 -
Na2S2O3 x 5 H2O 2.498 10.063
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Cultivation metadata from 7 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 7 strains
oxygen anaerobe 6 strains
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