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DSMZ

DSMZ 929 . RHODOVULUM IODOSUM AND R. ROBIGINOSUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Rhodovulum robiginosum DSM 12329

This strain uses the standard medium without modifications.

Growth conditions: more than 14 days anaerobic in light 28 °C

Main sol. 929 #

ml
Equipment needed: Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 26.40 g 26.4 451.745
MgCl2 x 6 H2O 5.70 g 5.7 28.037
MgSO4 x 7 H2O 6.80 g 6.8 27.589
CaCl2 x 2 H2O 1.50 g 1.5 10.203
KCl 0.66 g 0.66 8.853
KBr 0.09 g 0.09 0.756
Distilled water 1000.00 ml - -
1 Prepare the medium anaerobically under an atmosphere of N2:CO2 (8:2 vol:vol) and then add:
NaHCO3
(84 g/l autoclaved under a CO2 atmopshere) 		30.00 ml/l 2.52 29.998
KH2PO4 0.40 g/l 0.4 2.939
NH4Cl 0.25 g/l 0.25 4.674
Na2S2O3 0.50 mM 0.214 0.5
Trace elements 1.00 ml/l - -
Selenite/tungsate solution 1.00 ml/l - -
Vitamin solution 1.00 ml/l - -
Vitamin B12 solution 0.05 g/L 1.00 ml/l - -
Vitamin B1 solution 0.1 g/L 1.00 ml/l - -
Acetate 5.00 mM 0.295 5
FeSO4 10.00 mM 1.519 10
2 The pH is adjusted to 6.8
3 To the medium add 5 mM acetate and 10 mM FeSO4 (from a 1 M stock solution kept under nitrogen). When the iron is added to the medium a white floculant precipitate may appear. In the initial stages of growth the precipitate turns orange/brown as the ferrous ions are oxidised to ferrihydrite. Cultivation may take 2-3 weeks at a distance of 30 cm from a 25 W light bulb.

Trace elements #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 2.1 g 2.1 7.554
Na2-EDTA 5.2 g 5.2 15.467
H3BO3 30.0 mg 0.03 0.485
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
ZnSO4 x 7 H2O 144.0 mg 0.144 0.501
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Adjust the pH to 6.0

Selenite/tungsate solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SeO3 x 5 H2O 6 mg 0.006 0.023
Na2WO4 x 2 H2O 8 mg 0.008 0.024
NaOH 200 mg 0.2 5
Distilled water 1000 ml - -

Vitamin solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Para-aminobenzoic acid 4 mg 0.04 0.292
Biotin 1 mg 0.01 0.041
Nicotinate 10 mg 0.1 0.819
Pantothenate 5 mg 0.05 0.228
Pyridoxin 15 mg 0.15 0.887
Distilled water 100 ml - -

Vitamin B12 solution 0.05 g/L #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine 5 mg 0.05 0.037
Distilled water 100 ml - -

Vitamin B1 solution 0.1 g/L #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine 10 mg 0.1 0.377
Distilled water 100 ml - -

Metadata

Last modified: 11.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Anaerobic workbench
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2
Rhodovulum robiginosum DSM 12329

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 26.4 451.745
MgSO4 x 7 H2O 6.8 27.589
MgCl2 x 6 H2O 5.7 28.037
NaHCO3 2.52 29.998
FeSO4 1.519 10
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains