92a .
MODIFIED MEDIUM 92
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium 92 | 990 | ml | ||
|
|
Vitamin solution | 10 | ml | ||
| 1 Prepare medium 92 and subsequently add vitamins. Adjust pH to 7.0 - 7.2 | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.00 | mg |
|
|
Folic acid | 2.00 | mg |
|
|
Pyridoxine hydrochloride | 10.00 | mg |
|
|
Thiamine-HCl x 2 H2O | 5.00 | mg |
|
|
Riboflavin | 5.00 | mg |
|
|
Nicotinic acid | 5.00 | mg |
|
|
Calcium pantothenate | 5.00 | mg |
|
|
p-Aminobenzoic acid | 1.00 | mg |
|
|
Vitamin B12 | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |
Main sol. 92 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase soy broth | 30 | g | ||
|
|
Yeast extract | 3 | g | ||
|
|
Agar , if necessary | 15 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0 - 7.2. | |||||