959a .
MARINITOGA LITORALIS MEDIUM
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Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
6.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 26.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
NH4Cl | 1.00 | g | ||
|
|
KCl | 0.20 | g | ||
|
|
PIPES
(SIGMA) |
3.40 | g | ||
|
|
K2HPO4 | 0.35 | g | ||
|
|
KH2PO4 | 0.35 | g | ||
|
|
Na-acetate x 3 H2O | 2.72 | g | ||
|
|
Yeast extract
(OXOID) |
2.00 | g | ||
|
|
Trypticase peptone
(BD BBL) |
2.00 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
Maltose | 3.50 | g | ||
|
|
Na2S x 9 H2O | 0.50 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except maltose and sulfide, adjust pH to 5.8 - 6.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. The pH of the complete medium should be 6.0. | |||||