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961. THERMOVIBRIO MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(0.1% w/v)
0.5 ml 5.0e-4 0.002
2 1.0 g 1 9.891
3
(OXOID)
1.0 g 1 -
4 0.5 g 0.5 2.082
5 Synthetic seawater 1000.0 ml - -
Instructions:
  1. Dissolve resazurin in seawater, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into serum vials (e.g., 20 ml medium per 100 ml bottle) and autoclave. Add nitrate, yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 6.5, if necessary. After inoculation pressurize bottles to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
  2. Note: Alternatively, aged filtered seawater can be used instead of synthetic seawater.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 27.70 g 27.7 473.97
2 7.00 g 7 28.4
3 5.50 g 5.5 27.053
4 0.65 g 0.65 8.719
5 0.50 g 0.5 3.674
6 0.25 g 0.25 1.892
7 0.03 g 0.03 0.485
8 0.10 g 0.1 0.972
9 0.75 g 0.75 5.102
10 0.05 mg 5.0e-5 1.9e-4
11 15.00 mg 0.015 0.056
12 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % H2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 27.7 473.97
MgSO4 x 7 H2O 7 28.4
MgCl2 x 6 H2O 5.5 27.053
KNO3 1 9.891
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain

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