976 .
METHYLOPHAGA ALCALICA MEDIUM
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Equipment needed:
Autoclave
Final pH:
9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.00 | g | ||
|
|
KNO3 | 1.00 | g | ||
|
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MgSO4 x 7 H2O | 0.22 | g | ||
|
|
NaCl | 30.00 | g | ||
|
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Na2CO3 | 5.00 | g | ||
|
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Trace elements solution | 1.00 | ml | ||
|
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Distilled water | 1000.00 | ml | ||
|
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Methanol | 10.00 | ml | ||
| 1 Final pH 9.5 | |||||
| 2 Prepare the medium without the Na2CO3, which can be sterilised separately by autoclaving. 10 ml/l sterile methanol is added to the cooled medium. When preparing liquid media cool the mineral salts solution and Na2CO3 to room temperature before mixing. When preparing agar add 2.0 % agar to the mineral salts solution and autoclave. Cool the Na2CO3 stock solution and agar to 50-55°C before mixing. | |||||
Trace elements solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Ferric citrate | 30.0 | mg |
|
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CaCl2 x 2 H2O | 30.0 | mg |
|
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MgCl2 x 4 H2O | 5.0 | mg |
|
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ZnSO4 x 7 H2O | 5.0 | mg |
|
|
CuSO4 x 5 H2O | 0.5 | g |
|
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Distilled water | 1000.0 | ml |
|
|
Agar , for solid medium | 2.0 | % |