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986. PARAMALEDIVIBACTER MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Hungate tubes Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(SIGMA)
30.00 g 29.703 -
2 1.00 g 0.99 18.51
3 0.30 g 0.297 1.705
4 0.30 g 0.297 2.183
5 0.50 g 0.495 -
6 0.50 g 0.495 -
7
(0.1% w/v)
0.50 ml 5.0e-4 0.002
8 2.00 g 1.98 23.572
9 4.00 g 3.96 21.983
10 Vitamin solution 10.00 ml - -
11 0.25 g 0.248 1.409
12 0.25 g 0.248 1.031
13 1000.00 ml 988.324 54860.2
Instructions:
  1. Dissolve ingredients (except bicarbonate, glucose, vitamins, cysteine and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, glucose, sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.0.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Sea Salt 29.703 -
D-Glucose 3.96 21.983
NaHCO3 1.98 23.572
NH4Cl 0.99 18.51
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Cultivation metadata from 2 strains BacDive

pH 0 - 2 1 strain
pH 10 - 14 1 strain
pH 2 - 4 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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