Name: PERSEPHONELLA MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 996 #

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	29.00	g	29	496.235
NaOH	2.00	g	2	50.004
KCl	0.50	g	0.5	6.707
MgCl₂ x 6 H₂O	1.36	g	1.36	6.69
MgSO₄ x 7 H₂O	7.00	g	7	28.4
Na₂S₂O₃ x 5 H₂O	2.00	g	2	8.058
CaCl₂ x 2 H₂O	0.40	g	0.4	2.721
NH₄Cl	0.20	g	0.2	3.739
K₂HPO₄ x 3 H₂O	0.30	g	0.3	1.722
Trace element solution	10.00	ml	-	-
Distilled water	990.00	ml	-	-
1 Medium is prepared with anoxic water (under a CO₂-atmosphere) and the pH is adjusted to 6 with H₂SO₄ prior to autoclaving. Medium is dispensed under a CO₂-atmosphere into Bellco tubes (5 ml medium per 27 ml tube) and stoppered with butyl stoppers, capped and crimped. After autoclaving a white precipitate might be present, this precipitate can be redissolved by shaking the medium. It can take up to an hour before all the precipitate is dissolved.
2 3.8% O₂ is added to each tube (1 ml of O₂ per 27 ml tube) and after inoculation the tubes were pressurized with H₂ to 20 psi (or 138 kPa). Inoculum should be in mid-logarithmic phase (16-10 h after inoculation). Incubation is carried out without shaking at 70°C and both strains (DSM 14351 and DSM 14350) grow within 24 h.

Trace element solution #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na-EDTA x 2 H₂O	500	mg	0.5	1.487
CoCl₂ x 6 H₂O	150	mg	0.15	1.155
MnCl₂ x 4 H₂O	100	mg	0.1	0.505
FeSO₄ x 7 H₂O	100	mg	0.1	0.36
ZnCl₂	100	mg	0.1	0.734
AlCl3 x 6 H₂O	40	mg	0.04	0.166
Na₂O₄W x 2 H₂O	30	mg	0.03	-
CuCl	20	mg	0.02	0.202
NiSO₄ x 6 H₂O	20	mg	0.02	0.129
H₂SeO₃ (selenous acid)	10	mg	0.01	0.078
H₃BO₃	10	mg	0.01	0.162
Na₂MoO₄ x 2 H₂O	10	mg	0.01	0.041
Distilled water	1000	ml	-	-
1 Adjust pH of trace elements to 3.

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