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DSMZ

DSMZ 998 . MEDIUM FOR ROSEOSPIRA

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 998 #

ml
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.3 2.205
NH4Cl 0.50 g 0.5 9.348
CaCl2 x 2 H2O 0.05 g 0.05 0.34
MgCl2 x 6 H2O 1.00 g 1 4.919
MgSO4 x 7 H2O 0.50 g 0.5 2.029
NaCl 20.00 g 20 342.231
Trace element solution SL12 1.00 ml - -
Yeast extract 0.50 g 0.5 -
Distilled water 1000.00 ml - -
Vitamins V7 solution (Pfennig et Trüper, 1992) 1.00 ml - -
NaHCO3 0.15 %(w/v) 1.5 17.856
1 Media were autoclaved and cooled under N2/CO2 (90/10, v/v). Vitamin V7 solution (1 ml/l) and NaHCO3, (0.15% w/v) were then aseptically added to the medium. Final pH was adjusted to 6.8-7.0 and the medium was dispensed into sterile 50 ml screw-capped bottles. Organic substrates (5 mM Na-acetate and/or 5 mM di-Na-succinate) were added as substrates before use. In addition, for strains DSM 15114 and AT2115, Na2S x 9 H2O (0.02% w/v) were also added to the medium prior to utilisation. Pure cultures, grown in 50-ml screw-capped bottles were stored at +4°C in the dark for preservation.

Trace element solution SL12 #

1000
Solution occurs in 1 other media (first defined in Medium 998).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.0 ml - -
H3BO3 300.0 mg 0.3 4.851
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 2 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
1 Sterilized by filtration.

Vitamins V7 solution (Pfennig et Trüper, 1992) #

1000
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000 ml - -
Biotine 2 mg 0.002 0.008
p-Aminobenzoate 10 mg 0.01 0.073
Thiamine 10 mg 0.01 0.038
Pantothenate 5 mg 0.005 0.023
Pyridoxamine 50 mg 0.05 0.297
Vitamine B12 20 mg 0.02 0.015
Nicotinate 20 mg 0.02 0.164
1 Sterilized by filtration.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Filter
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Gas composition

  • 90 % N2
  • 10 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
NH4Cl 0.5 9.348
MgSO4 x 7 H2O 0.5 2.029
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Cultivation metadata from 1 strains BacDive

phyla 1 2 strains
temperature mesophilic 1 strain