110 .
ASN-III
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 25.00 | g | ||
|
|
MgSO4 x 7 H2O | 3.50 | g | ||
|
|
MgCl2 x 6 H2O | 2.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.50 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
Citric acid | 3.00 | mg | ||
|
|
Ferric ammonium citrate | 3.00 | mg | ||
|
|
EDTA | 0.50 | mg | ||
|
|
A-5 Trace Metals | 1.00 | ml | ||
|
|
NaNO3 | 0.75 | g | ||
|
|
K2HPO4 x 3 H2O | 0.75 | g | ||
|
|
Na2CO3 | 0.02 | g | ||
|
|
Vitamin B12 | 10.00 | mg | ||
|
|
Agar
(Noble) |
10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.3. Filter-sterilize and dispense into appropriate vessel. | |||||
A-5 Trace Metals #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
H3BO3 | 2.860 | g |
|
|
MnCl2 x 4 H2O | 1.810 | g |
|
|
ZnSO4 x 7 H2O | 0.222 | g |
|
|
Na2MoO4 x 2 H2O | 0.039 | g |
|
|
CuSO4 x 5 H2O | 0.079 | g |
|
|
Co(NO3)2 x 6 H2O | 0.049 | g |
|
|
Distilled water | 1000.000 | ml |