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CCAP 29 . E26 + Biotin:ANT

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C29 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
E26 + biotin medium 500 ml - -
Antia's medium (ANT) 500 ml - -
1 E26 + biotin medium and Antia's medium are made up separately then mixed 1:1.

E26 + biotin medium #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Part 1 500 ml - -
Natural sea water
(filtered and autoclaved) 		500 ml - -
1 This medium is made up in 2 parts which are autoclaved separately and mixed aseptically, (1:1), when cool. This is to avoid precipitation.
2 Alternatively, use 17.5 g of "Ultramarine Synthetica" sea salts in 500 ml of distilled water.

Part 1 #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
SE1 (Soil Extract 1) 50.00 ml - -
KNO3 0.10 g 0.2 1.978
K2HPO4 0.01 g 0.02 0.115
MgSO4 x 7 H2O 0.01 g 0.02 0.081
Cyanocobalamine 0.10 μg - -
Thiamine HCl 50.00 μg - -
Biotin 0.10 μg - -
Distilled water 500.00 ml - -
1 Distilled water to 500 ml. Autoclave

SE1 (Soil Extract 1) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air-dried) 		trace - -
Distilled water trace - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
3 Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Antia's medium (ANT) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 0.0500 g 0.05 0.495
NaH2PO4 x 2 H2O 0.0078 g 0.008 0.065
Tris 1.0000 g 1 8.255
Glycine 0.3000 g 0.3 3.996
Trace metals stock solution (chelated) 2.5000 ml - -
Thiamine HCl 500.0000 μg - -
Cyanocobalamine 2.0000 μg - -
Biotine 1.0000 μg - -
Natural sea water
(filtered) 		800.0000 ml - -
1 Make up to 1 litre with distilled water and autoclave at 15 psi. Final pH should be 7.6 -­ 7.8.

Trace metals stock solution (chelated) #

1000
Solution occurs in 2 other media (first defined in Medium C3).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 3.2400 g 3.24 9.637
FeCl3 x 6 H2O 1.0800 g 1.08 3.996
MnSO4 x 4 H2O 0.4500 g 0.45 2.017
ZnSO4 x 7 H2O 0.2300 g 0.23 0.8
Na2MoO4 x 2 H2O 0.0970 g 0.097 0.401
CuSO4 x 5 H2O 0.0100 g 0.01 0.04
CoSO4 x 7 H2O 0.0056 g 0.006 0.036
1 Make up to 1 litre with distilled water and adjust pH to 7.6 ­- 7.8 with dilute HCl or NaOH. Store frozen.

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Tris 0.5 4.127
Glycine 0.15 1.998
KNO3 0.025 0.247
K2HPO4 0.005 0.029
MgSO4 x 7 H2O 0.005 0.02
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