88 .
R75S:NSW
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Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Medium C87 | 600 | ml | ||
|
|
NSW (Natural Seawater) | 300 | ml | ||
| 1 2:1 mixture | |||||
| 2 See separate recipes below. Autoclave separately. Mix aseptically when cool. | |||||
Mail sol. C87 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Natural sea water
(filtered) |
750 | ml | ||
|
|
Deionized water | 250 | ml | ||
|
|
Rye grass
(dried, milled) |
1 | g | ||
| 1 Bring 75% seawater to the boil, add the powdered rye grass and keep boiling for 5 minutes. Allow to cool and restore volume to 1 litre with deionized water. Filter through Whatman GF/C paper. Autoclave at 15 psi for 15 minutes. | |||||
NSW (Natural Seawater) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Natural sea water
(filtered) |
1000 | ml | ||
| 1 Filter natural seawater through GF/C filters and autoclave at 15 psi for 15 minutes. | |||||