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CCAP 93 . SES:MP

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C93 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mail sol. C92 750 ml - -
Medium C57 250 ml - -
1 3:1 mixture
2 See separate recipes. Autoclave separately. Mix aseptically.

Mail sol. C92 #

1000
Solution occurs in 1 other media (first defined in Medium C93).
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(1 g/l stock solution) 		20 ml - -
MgSO4 x 7 H2O
(1 g/l stock solution) 		20 ml - -
KNO3
(10 g/l stock solution) 		20 ml - -
SE2 (Soil Extract 2) 100 ml - -
Deionized water 1000 ml - -
1 Make up to 1 litre with deionized water and autoclave at 15 psi for 15 minutes.
2 At the CCAP, SE1 is used for marine algae, SE2 for freshwater and terrestrial protozoa.

SE2 (Soil Extract 2) #

1000
Solution occurs in 13 other media (first defined in Medium C12).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air-dried) 		trace - -
Distilled water trace - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
3 Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

SE1 (Soil Extract 1) #

1000
Solution occurs in 3 other media (first defined in Medium C4).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Soil
(air-dried) 		trace - -
Distilled water trace - -
1 Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
2 A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
3 Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Medium C57 #

1000
Solution occurs in 3 other media (first defined in Medium C57).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ca(NO3)2 x 4 H2O
(20 g/100 ml stock solution) 		1 ml - -
MgSO4 x 7 H2O
(2 g/100 ml stock solution) 		1 ml - -
Na2HPO4 x 2 H2O
(2 g/100 ml stock solution) 		1 ml - -
KCl
(2.6 g/100 ml stock solution) 		1 ml - -
Stock solution 1 1 ml - -
Deionized water 1000 ml - -
1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.

Stock solution 1 #

100
Solution occurs in 3 other media (first defined in Medium C57).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe SO4 x 7 H2O 0.2 g 2 7.194
H2SO4
(concentrated) 		0.1 ml - -

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Fe SO4 x 7 H2O 5.0e-4 0.002
K2HPO4 - -
KCl - -
Na2HPO4 x 2 H2O - -
Ca(NO3)2 x 4 H2O - -
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