CCAP 97 . SNA/5

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Equipment needed:

Final pH: n.d.

Select a strain (optional):

ml
Compound Amount Unit
Nutrient agar
(Oxoid CM3)
28 g
Natural sea water
(filtered)
200 ml
Deionized water 800 ml
1 Make up to 1 litre with deionized water. Steam for 30 minutes, ensuring homogeneity, then dispense as required. Autoclave at 15 psi and slope (tubes) prior to cooling.
2 Alternatively use "Ultramarine" Synthetica sea salts 17.5 g