99 .
S/W
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Equipment needed:
Final pH:
7.0 - 8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Soil
(air-dried, sieved, garden loam) |
trace | g | ||
| 1 Put a layer about 1 cm deep of air-dried, sieved good calcareous garden loam into a test tube or jar. (The use of mud from rivers or ponds is rarely satisfactory.) Carefully add deionised water to a depth of 7 to 10 cm, plug or cover, and steam for one hour or autoclave for 15 minutes at 15 psi (longer for larger vessels) on each of 2 consecutive days; further sterilisation is not needed. Allow to stand for a further day before inoculating, when the pH should be between 7.0 - 8.0. | |||||