1020 .
DESULFOCARBO INDIANENSIS MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2SO4 | 2.000 | g | ||
|
|
NaCl | 8.800 | g | ||
|
|
MgCl2 x 6 H2O | 4.600 | g | ||
|
|
KCl | 0.340 | g | ||
|
|
NH4Cl | 0.250 | g | ||
|
|
KH2PO4 | 0.140 | g | ||
|
|
CaCl2 x 2 H2O | 0.140 | g | ||
|
|
NiCl2 x 6 H2O | 9.300 | mg | ||
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 2.700 | mg | ||
|
|
Na2SeO3 x 5 H2O | 0.260 | mg | ||
|
|
Na2WO4 x 2 H2O | 0.084 | mg | ||
|
|
Trace element solution | 10.000 | ml | ||
|
|
Trace vitamins | 10.000 | ml | ||
|
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Sodium benzoate | 0.360 | g | ||
|
|
Resazurin | 1.000 | mg | ||
|
|
Distilled water | 1000.000 | ml | ||
| 1 Mix components thoroughly and distribute the medium into culture vessels (e.g., 10 ml medium in Balch-type tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions: | |||||
|
|
NaHCO3
(filter-sterilized, 8%) |
60.000 | ml | ||
|
|
Na2S x 9 H2O
(autoclaved, 5%) |
5.000 | ml | ||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |