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JCM 1021 . MM4Y MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1021 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
A-solution 100.00 ml - -
B-solution 100.00 ml - -
Trace element solution 0.02 ml - -
Yeast extract
(BD-Difco) 		0.05 g 0.05 -
Agar
(Purified) 		15.00 g 15 44.599
Distilled water 800.00 ml - -

A-solution #

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 39.600 g - -
MgSO4 x 7 H2O 2.460 g - -
FeSO4 x 7 H2O 55.600 mg - -
CaCl2 x 2 H2O 0.147 g - -
NaCl 5.000 g - -
1 Add components to distilled water and bring volume to 1.0 L.

B-solution #

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 55.51 g - -
KH2PO4 12.93 g - -
1 Add components to distilled water and bring volume to 1.0 L. Sterilize by autoclaving for storage.

Trace element solution #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2MoO4 x 2 H2O 0.017 g 0.17 0.703
ZnSO4 x 7 H2O 0.080 g 0.8 2.782
CuSO4 x 5 H2O 5.000 mg 0.05 0.2
H3BO3 0.010 g 0.1 1.617
MnSO4 x n H2O 0.060 g 0.6 -
CoCl2 x 6 H2O 0.010 g 0.1 0.77
NiSO4 x 6 H2O 0.010 g 0.1 0.646
Distilled water 100.000 ml - -
1 Sterilize by filtration for storage.

Metadata

Last modified: 29.10.21
Source: JCM
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Yeast extract 0.05 -
ZnSO4 x 7 H2O 1.6e-5 5.6e-5
MnSO4 x n H2O 1.2e-5 -
Na2MoO4 x 2 H2O 3.4e-6 1.4e-5
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