1047 .
MARINITOGA MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Sea Salt
(Sigma) |
30.0 | g | ||
|
|
PIPES | 6.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Tryptone
(BD-Difco) |
1.0 | g | ||
|
|
Resazurin | 0.5 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix components and adjust pH to 7.0. Bubble the medium with N2 for 5 min, then distribute the medium into culture vessels under N2, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions aseptically and anaerobically (autoclaved and stored under a N2 gas atmosphere): | |||||
|
|
Glucose
(1.0 M) |
14.0 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
10.0 | ml | ||
|
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | ||