J1061
1061 .
HALOBACTERIA MH4.2 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1061 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sodium pyruvate | 1.00 | g | 9.901 | 89.976 | |
| Trisodium citrate x 2 H2O | 1.00 | g | 9.901 | - | |
|
Yeast extract
(BD-Difco) |
2.00 | g | 19.802 | - | |
|
Tryptone
(BD-Difco) |
2.00 | g | 19.802 | 278.587 | |
| K2HPO4 | 0.60 | g | 5.941 | 34.107 | |
| NH4Cl | 0.60 | g | 5.941 | 111.06 | |
| NaCl | 200.00 | g | 1980.2 | 33884.3 | |
| MgSO4 x 7 H2O | 0.50 | g | 4.951 | 20.085 | |
| K2SO4 | 5.00 | g | 49.505 | 284.087 | |
| SL-6 trace element solution | 1.00 | ml | - | - | |
| FeCl2 x 4 H2O | 36.00 | mg | 0.356 | 1.793 | |
| MnCl2 x 4 H2O | 0.36 | mg | 0.004 | - | |
|
Tris-HCl
(pH 8.0, 0.5 M) |
100.00 | ml | - | - | |
| 1 Add components to distilled water and bring volume to 1.0 L. For preparation of solid medium, add 20.0 g agar. | |||||
Solution occurs in 10
other media (first defined in Medium J167).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
| H3BO3 | 0.30 | g | 0.3 | 4.851 | |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 3.6. | |||||
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 1980.2 | 33884.3 |
| K2SO4 | 49.505 | 284.087 |
| Tryptone | 19.802 | 278.587 |
| Yeast extract | 19.802 | - |
| Sodium pyruvate | 9.901 | 89.976 |
| Show more | ||
