1063 .
SEA SALTS YEAST EXTRACT GLUCOSE MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Sea Salt
(Sigma) |
30.00 | g | ||
|
|
KH2PO4 | 0.15 | g | ||
|
|
K2HPO4 | 0.15 | g | ||
|
|
NH4Cl | 2.00 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
Glucose | 5.00 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
NaHCO3 | 1.00 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components except NaHCO3 and adjust pH to 7.0. Bring to a boil, cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream and add NaHCO3. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. | |||||