1082 .
HALALKALIARCHAEUM MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Basal mineral NaCl medium
(sterilized, see Medium No. 1079) |
937.5 | ml | ||
|
|
Soda based mineral medium
(sterilized) |
62.5 | ml | ||
| 1 To complete the medium, aseptically add the following solution (autoclaved or *filter-sterilized): | |||||
|
|
Sodium pyruvate
(*, 2 M) |
5.0 | ml | ||
|
|
Trace vitamins (see Medium No. 197)
(*) |
10.0 | ml | ||
|
|
Yeast extract
(10%) |
0.2 | ml | ||
Soda based mineral medium #
4
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2CO3 | 185 | g | ||
|
|
NaHCO3 | 35 | g | ||
|
|
NaCl | 16 | g | ||
|
|
K2HPO4 | 1 | g | ||
|
|
KCl | 5 | g | ||
| 1 Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved): | |||||
|
|
NH4Cl
(4 M) |
1 | ml | ||
|
|
Selenite-tungstate solution | 1 | ml | ||
|
|
Trace element solution | 1 | ml | ||
|
|
MgCl2
(1 M) |
1 | ml | ||