1088 .
0.3 x HYPHOMICROBIUM MEDIUM
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Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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K2HPO4 | 0.85 | g | ||
|
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NaH2PO4 | 0.75 | g | ||
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(NH4)2SO4 | 0.20 | g | ||
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MgSO4 x 7 H2O | 0.07 | g | ||
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Visniac trace elements | 1.00 | ml | ||
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Trace vitamins | 2.00 | ml | ||
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Methanol | 2.00 | ml | ||
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Agar | 15.00 | g | ||
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Distilled water | 1000.00 | ml | ||
| 1 After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol. | |||||
Trace vitamins #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 125.0 | mg |
|
|
Thiamine HCl | 25.0 | mg |
|
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Folic acid | 12.5 | mg |
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Ascorbic acid | 40.0 | mg |
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Riboflavin | 25.0 | mg |
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Niacin | 25.0 | mg |
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Pantothenic acid | 25.0 | mg |
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Distilled water | 500.0 | ml |
Visniac trace elements #
500
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2-EDTA | 5.000 | g |
|
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ZnSO4 x 7 H2O | 2.200 | g |
|
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CaCl2 x 2 H2O | 0.733 | g |
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MnCl2 x 4 H2O | 0.506 | g |
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FeSO4 x 7 H2O | 0.499 | g |
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(NH4)6Mo7O24 x 4 H2O | 0.110 | g |
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CuSO4 x 5 H2O | 0.157 | g |
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CoCl2 x 6 H2O | 0.161 | g |
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Distilled water | 500.000 | ml |