1098 .
BSM-RC MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 3.620 | g | ||
|
|
K2HPO4 | 0.260 | g | ||
|
|
NH4Cl | 0.075 | g | ||
|
|
Na2SO4 | 0.050 | g | ||
|
|
NaNO3 | 0.850 | g | ||
|
|
L-Sodium lactate | 2.240 | g | ||
|
|
Yeast extract | 0.200 | g | ||
|
|
Tryptone | 0.500 | g | ||
|
|
FeCl2 solution | 1.000 | ml | ||
|
|
Trace element solution | 1.000 | ml | ||
|
|
Trace vitamins | 10.000 | ml | ||
|
|
Distilled water | 935.500 | ml | ||
| 1 Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, add the following solution (filter-sterilized): | |||||
|
|
NaHCO3
(8%) |
52.500 | ml | ||
| 2 Aseptically and anaerobically distribute the medium into culture vessels under a N2 atmosphere and add H2 at 5% volume of the gas phase. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgCl2 x 6 H2O | 3 | g |
|
|
CaCl2 x 2 H2O | 100 | mg |
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |