MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 1100 . CTM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1100 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (50 x stock) 20.0 ml - -
Solution 2 3.0 ml - -
Solution 3 2.0 ml - -
Solution 4 2.5 ml - -
HEPES 2.4 g 85.714 359.676
1 CTM medium basis:
2 Mix all ingredients in 1.0 L medium bottle. Add distilled water close to 1.0 L. Adjust pH to 7.0 with 2 M KOH and fill up to 1.0 L. Autoclave at 121C for 40 min. After autoclaving, seal medium bottle immediately in order to avoid oxygenation. Cool down to around 70C. Add 30 ml filter-sterilized Mixed solution (see below) to the autoclaved CTM medium basis for completing the CTM medium.

Solution 1 (50 x stock) #

1051
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 3.75 g 3.568 14.476
CaCl2 x 2 H2O 1.80 g 1.713 11.65
Citric acid 0.30 g 0.285 1.486
Na2-EDTA
(pH 8.0, 0.25 M) 		0.60 ml - -
Trace metal solution 50.00 ml - -
Distilled water 1000.00 ml - -

Trace metal solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 2.860 g 2.86 46.248
MnCl2 x 4 H2O 1.810 g 1.81 9.146
ZnSO4 x 7 H2O 0.222 g 0.222 0.772
Na2MoO4 x 2 H2O 0.390 g 0.39 1.612
CuSO4 x 5 H2O 0.079 g 0.079 0.316
Co(NO3)2 x 6 H2O 0.049 g 0.049 0.168
Distilled water 1000.000 ml - -

Solution 2 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 15.3 g 15.3 87.842
Distilled water 1000.0 ml - -

Solution 3 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Ferric ammonium citrate 12 g 12 45.805
Distilled water 1000 ml - -

Solution 4 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
2-Oxoglutarate 14.6 g 14.6 101.331
Distilled water 1000.0 ml - -

Mixed solution #

11
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 0.625 g 56.818 676.351
Sodium thioglycolate 0.125 g 11.364 99.594
Solution 5 0.500 ml - -
Solution 6 10.000 ml - -
Vitamin solution A (1000 x stock) 0.250 ml - -
Vitamin solution B (1000 x stock) 0.250 ml - -
1 Add distilled water to bring final volume to 30 ml.

Solution 5 #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone
(BD-Difco) 		10 g 100 -
Distilled water 100 ml - -

Solution 6 #

400
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Alanine 100 mg 0.25 2.806
L-Arginine 100 mg 0.25 1.435
L-Asparagine 100 mg 0.25 1.892
L-Aspartic acid 100 mg 0.25 1.878
L-Cysteine 100 mg 0.25 2.063
L-Glutamic acid 100 mg 0.25 1.699
L-Glutamine 100 mg 0.25 1.711
Glycine 100 mg 0.25 3.33
L-Histidine 100 mg 0.25 1.611
L-Isoleucine 100 mg 0.25 1.906
L-Leucine 100 mg 0.25 1.906
L-Lysine 100 mg 0.25 1.71
L-Methionine 100 mg 0.25 1.675
L-Phenylalanine 100 mg 0.25 1.513
L-Proline 100 mg 0.25 2.171
L-Serine 100 mg 0.25 2.379
L-Threonine 100 mg 0.25 2.099
L-Tryptophan 100 mg 0.25 1.224
L-Tyrosine 100 mg 0.25 1.38
L-Valine 100 mg 0.25 2.134
Distilled water 400 ml - -

Vitamin solution A (1000 x stock) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 10 mg 0.1 0.409
Riboflavin 10 mg 0.1 0.266
Thiamine HCl 100 mg 1 2.965
Thiamine pyrophosphate 100 mg 1 2.17
L-Ascorbic acid 100 mg 1 5.678
Calcium pantothenate 100 mg 1 2.098
Folic acid 100 mg 1 2.266
Nicotinamide 100 mg 1 8.189
Nicotinic acid 100 mg 1 8.123
p-Aminobenzoic acid 100 mg 1 7.292
Pyridoxine hydrochloride 100 mg 1 4.863
Lipoic acid 100 mg 1 4.847
beta-NAD 100 mg 1 1.505
Potassium phosphate buffer
(pH 7.2, 10 mM) 		100 ml - 1

Vitamin solution B (1000 x stock) #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 1 0.738
Distilled water 100 ml - -

Metadata

Last modified: 03.04.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
HEPES 85.714 359.676
MgSO4 x 7 H2O 2.549 10.34
K2HPO4 1.639 9.412
2-Oxoglutarate 1.304 9.047
CaCl2 x 2 H2O 1.223 8.321
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature thermophilic 1 strain