1106 .
MARINE METHANOGENIC MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 22.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.14 | g | ||
|
|
KCl | 0.34 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
MgCl2 x 6 H2O | 2.75 | g | ||
|
|
K2HPO4 | 0.14 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.20 | g | ||
|
|
Trace mineral solution | 10.00 | ml | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 930.00 | ml | ||
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
NaHCO3
(*, 8%) |
30.00 | ml | ||
|
|
Trace vitamins
(*) |
10.00 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
10.00 | ml | ||
|
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | ||
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Trace minerals | 1000.00 | ml |
|
|
NiCl2 x 6 H2O | 0.03 | g |
|
|
Na2SeO3 x 5 H2O | 0.30 | mg |
|
|
Na2WO4 x 2 H2O | 0.40 | mg |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |