1155 .
DESULFOOBULBUS OLIGOTROPHICUS MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.50 | g | ||
|
|
K2HPO4 | 0.50 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
NaCl | 5.00 | g | ||
|
|
Na2SO4 | 2.84 | g | ||
|
|
MgCl2 x 6 H2O | 0.30 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
KCl | 0.40 | g | ||
|
|
Trace element solution
(see Medium No. 294) |
1.00 | ml | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 955.00 | ml | ||
| 1 Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
NaHCO3
(*, 8%) |
25.00 | ml | ||
|
|
Sodium propionate
(1 M) |
20.00 | ml | ||
|
|
Trace vitamins
(*) |
1.00 | ml | ||
| 2 Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2): | |||||
|
|
Na2S x 9 H2O
(5%) |
8.00 | ml | ||
| 3 Readjust pH to 7.6, if necessary. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |