J1168
1168 .
LB (LURIA-BERTANI) AGAR WITH KANAMYCIN AND RIFAMPICIN
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1168 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Tryptone
(BD-Difco) |
10 | g | 10 | 140.687 | |
|
Yeast extract
(BD-Difco) |
5 | g | 5 | - | |
| NaCl | 10 | g | 10 | 171.116 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 After autoclaving, aseptically add the following antibiotics (filter-sterilized): | |||||
|
Kanamycin sulfate
(dissolved in distilled water) |
50 | mg | 0.05 | 0.086 | |
|
Rifampicin
(dissolved in methanol) |
25 | mg | 0.025 | 0.03 | |
| 2 Adjust pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |