1180 .
CELLULOLYTIC NATRONOARCHAEA MEDIUM
What is this page?
Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 128.00 | g | ||
|
|
Na2CO3 | 95.00 | g | ||
|
|
NaHCO3 | 15.00 | g | ||
|
|
KCl | 2.50 | g | ||
|
|
K2HPO4 | 1.75 | g | ||
| 1 Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
NH4Cl
(10%) |
1.25 | ml | ||
|
|
MgSO4
(1 M) |
1.00 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Trace vitamins
(*) |
5.00 | ml | ||
|
|
Yeast extract
(10%) |
0.20 | ml | ||
|
|
Cellobiose
(10%) |
20.00 | ml | ||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5.00 | g | ||
|
|
FeSO4 x 7 H2O | 2.00 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 3.6. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |