J1210
1210 .
MARINITOGA HYDROGENITOLERANS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1210 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Sea Salt
(Sigma) |
30.0 | g | 29.014 | - | |
| MES | 4.0 | g | 3.868 | 19.814 | |
|
Yeast extract
(Oxoid) |
1.0 | g | 0.967 | - | |
|
Tryptone
(BD-Difco) |
1.0 | g | 0.967 | 13.606 | |
| Resazurin | 0.5 | mg | 4.8e-4 | 0.002 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components and adjust pH to 6.0. Bubble the medium with N2 for 5 min, then distribute the medium into culture vessels under N2, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions aseptically and anaerobically (autoclaved and stored under a N2 gas atmosphere): | |||||
|
Glucose
(1.0 M) |
14.0 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.0 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sea Salt | 29.014 | - |
| MES | 3.868 | 19.814 |
| Tryptone | 0.967 | 13.606 |
| Yeast extract | 0.967 | - |
| Resazurin | 4.8e-4 | 0.002 |
| Show more | ||
