1247 .
R2A MEDIUM WITH 25% SALT
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Equipment needed:
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(BD-Difco) |
0.50 | g | ||
|
|
Proteose peptone no. 3
(BD-Difco) |
0.50 | g | ||
|
|
Casamino acids
(BD-Difco) |
0.50 | g | ||
|
|
Glucose | 0.50 | g | ||
|
|
Starch
(soluble) |
0.50 | g | ||
|
|
Sodium pyruvate | 0.30 | g | ||
|
|
K2HPO4 | 0.30 | g | ||
|
|
MgSO4 x 7 H2O | 0.05 | g | ||
|
|
Sea water
(see below, 30%) |
833.00 | ml | ||
|
|
Distilled water | 167.00 | ml | ||
| 1 30% Seawater solution: | |||||
|
|
NaCl | 234.00 | g | ||
|
|
MgCl2 x 6 H2O | 39.00 | g | ||
|
|
MgSO4 x 7 H2O | 61.00 | g | ||
|
|
KCl | 6.00 | g | ||
|
|
CaCl2 x 2 H2O | 1.30 | g | ||
|
|
NaBr | 0.70 | g | ||
|
|
NaHCO3 | 0.20 | g | ||
| 2 Adjust pH to 7.5. | |||||
| 3 Add components to distilled water and bring volume to 1.0 L. | |||||