J1268
1268 .
CAPROICIPRODUCENS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1268 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 2.000 | g | 2 | 15.136 | |
| K2HPO4 | 1.000 | g | 1 | 5.741 | |
| KH2PO4 | 0.500 | g | 0.5 | 3.674 | |
| FeSO4 x 7 H2O | 0.015 | g | 0.015 | 0.054 | |
| MgSO4 x 7 H2O | 0.100 | g | 0.1 | 0.406 | |
| MnSO4 x n H2O | 0.010 | g | 0.01 | - | |
| CaCl2 x 2 H2O | 0.010 | g | 0.01 | 0.068 | |
| ZnSO4 x 7 H2O | 2.000 | mg | 0.002 | 0.007 | |
| CoCl2 x 6 H2O | 2.000 | mg | 0.002 | 0.015 | |
| Distilled water | 645.000 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 6.5. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved): | |||||
|
Yeast extract
(10%) |
100.000 | ml | - | - | |
|
Tryptone
(10%) |
100.000 | ml | - | - | |
|
Glucose
(1.0 M) |
100.000 | ml | - | - | |
|
Sodium acetate
(1.0 M) |
20.000 | ml | - | - | |
|
Sodium butyrate
(1.0 M) |
35.000 | ml | - | - | |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |