1273 .
0.01% LB SEAWATER MEDIUM
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Equipment needed:
Autoclave
Final pH:
8.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaNO3 | 120.0 | mg | ||
|
|
K2HPO4 | 5.0 | mg | ||
|
|
Vitamin B12 | 1.0 | μg | ||
|
|
Biotin | 1.0 | μg | ||
|
|
Thiamine HCl | 100.0 | μg | ||
|
|
Fe(III)-EDTA | 259.0 | μg | ||
|
|
Mn(II) x EDTA | 332.0 | μg | ||
|
|
Tris(hydroxymethyl)aminomethane | 1.0 | g | ||
|
|
LB Broth
, Lennox
(BD-Difco) |
0.1 | g | ||
|
|
Sea water | 1000.0 | ml | ||
| 1 Mix components thoroughly, adjust pH to 8.0 with HCl and autoclave. | |||||
| 2 Comment: Artificial sea water (such as artificial sea water in Medium No. 1118) can be used instead of natural sea water. | |||||