1283 .
SIUC-3 MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HEPES | 3.60 | g | ||
|
|
Na2SO4 | 3.24 | g | ||
|
|
K2HPO4 | 0.17 | g | ||
|
|
MgCl2 x 6 H2O | 0.40 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
NH4Cl | 0.30 | g | ||
|
|
NaHCO3 | 0.25 | g | ||
|
|
H3BO3 | 6.00 | mg | ||
|
|
Wolfe's mineral solution | 1.00 | ml | ||
|
|
Sodium formate | 0.68 | g | ||
|
|
Yeast extract | 0.10 | g | ||
|
|
Resazurin | 0.20 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly and adjust pH to 6.5. Distribute the medium into culture vessels (e.g., 25 ml in 120 ml-serum bottles) under a stream of a N2 gas, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved) : | |||||
|
|
Na2S x 9 H2O
(5%) |
10.00 | ml | ||
Wolfe's mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Trace minerals | 1000.00 | ml |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2SeO3 | 1.00 | mg |
|
|
Na2WO4 x 2 H2O | 0.01 | g |
Trace minerals #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 1.50 | g | ||
|
|
MgSO4 x 7 H2O | 3.00 | g | ||
|
|
MnSO4 x n H2O | 0.50 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
CoSO4 x 7 H2O | 0.10 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
AlK(SO4)2 | 0.01 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||