1291 .
GS2 MEDIUM WITH CELLOBIOSE
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| 1 Mix components thoroughly and adjust pH to 7.2 with NaOH. Bring to a boil, then cool down and distribute into culture vessels under a N2 gas stream, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add the following solution (autoclaved): | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.50 | g | ||
|
|
K2HPO4 | 2.90 | g | ||
|
|
Urea | 2.10 | g | ||
|
|
MOPS | 10.00 | g | ||
|
|
Yeast extract | 6.00 | g | ||
|
|
Trisodium citrate x 2 H2O | 2.90 | g | ||
|
|
L-Cysteine HCl x H2O | 0.25 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
GS2 salt solution | 10.00 | ml | ||
|
|
Cellobiose
(0.2 M) |
8.00 | ml | ||
GS2 salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MgCl2 x 6 H2O | 100.0 | g |
|
|
CaCl2 x 2 H2O | 15.0 | g |
|
|
FeSO4
(1.25%) |
0.1 | ml |
|
|
Distilled water | 1000.0 | ml |