J1303
1303 .
UREAPLASMA MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1303 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
PPLO broth
(BD-Difco 255420, without Crystal Violet) |
2.1 | g | 10.5 | - | |
|
Phenol red
(see below, 1%) |
200.0 | �l | - | - | |
| Hipolypepton | 1.0 | g | 5 | - | |
| Distilled water | 85.0 | ml | - | - | |
| 1 Mix components, adjust pH to 6.0-6.2. After autoclaving and then cooling to 50-55C, aseptically add the following solutions (filter-sterilized). | |||||
|
Bovine calf serum
(heat-inactivated) |
5.0 | ml | - | - | |
|
Urea
(see below, 1%, 4 % yeast extract) |
10.0 | ml | - | - | |
| 2 1% Urea - 4% yeast extract solution: | |||||
| Urea | 1.0 | g | 5 | 83.256 | |
| Yeast extract | 4.0 | g | 20 | - | |
| Distilled water | 100.0 | ml | - | - | |
| 3 1% Phenol red solution: | |||||
| 4 Dissolve 1.0 g phenol red first in 10-20 ml of 0.1 N NaOH. Then adjust volume to 100 ml. After filter-sterilizing, store at 4C. | |||||
| 5 Mix components, sterilize by filtration through a 0.22 �m filter and store at 4C. | |||||
| 6 Dispense the medium into sterilized plastic tubes (e.g., 5 ml medium in 15 ml of plastic tubes) and add 100-200 �l of culture solution. Incubate the cultures at 37C for 1-3 days. When the color of the medium turns from yellow to orange or red, subculture into the new medium. After color change, the strain can be stored at 4C for 1-2 weeks. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 6.1 |
| Equipment needed: | Filter |
| Edit in Medium builder | |