J1325
1325 .
ANAEROBIC DOUBLE-STRENGTH R2A MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1325 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(BD-Difco) |
1.0 | g | 1 | - | |
|
Proteose peptone no. 3
(BD-Difco) |
1.0 | g | 1 | - | |
|
Casamino acids
(BD-Difco) |
1.0 | g | 1 | - | |
| Glucose | 1.0 | g | 1 | 5.551 | |
|
Starch
(soluble) |
1.0 | g | 1 | - | |
| Sodium pyruvate | 0.6 | g | 0.6 | 5.453 | |
| K2HPO4 | 0.6 | g | 0.6 | 3.445 | |
| MgSO4 x 7 H2O | 0.1 | g | 0.1 | 0.406 | |
| L-Cysteine HCl x H2O | 0.2 | g | 0.2 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.2, distribute the medium into culture vessels under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |