1328 .
PROLIXIBACTER MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 18.00 | g | ||
|
|
MgCl2 x 6 H2O | 2.60 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
KH2PO4 | 0.20 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
NaNO3 | 0.85 | g | ||
|
|
Glucose | 1.80 | g | ||
|
|
Yeast extract
(BD-Difco) |
1.00 | g | ||
|
|
Trace minerals
(see Medium No. 243) |
1.00 | ml | ||
|
|
Trace vitamins | 1.00 | ml | ||
|
|
NaHCO3 | 2.52 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components, and adjust pH to 7.0. Dispense the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers, and autoclave. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): | |||||
|
|
Na2S x 9 H2O
(5%) |
10.00 | ml | ||
|
|
L-Cysteine HCl x H2O
(5%) |
10.00 | ml | ||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |