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JCM 133 . MEDIUM 10

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J133 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone
(BD-BBL) 		2.0 g 1.91 -
Yeast extract
(BD-Difco) 		0.5 g 0.478 -
Hemin
(0.2%) 		1.0 ml - -
VFA solution 3.1 ml - -
Glucose 0.5 g 0.478 2.651
Cellobiose 0.5 g 0.478 1.395
Starch
(soluble) 		0.5 g 0.478 -
Salt solution No. 1 37.5 ml - -
Salt solution No. 2 37.5 ml - -
Agar 18.0 g 17.192 51.116
Resazurin
(0.1%) 		0.5 ml - -
Toray silicone SH 5535
(10%) 		5.0 ml - -
L-Cysteine HCl x H2O
(5%) 		10.0 ml - -
L-Ascorbic acid
(25%) 		2.0 ml - -
Na2SO3
(4%) 		100.0 ml - -
Distilled water 850.0 ml - -
1 Adjust pH to 6.7-6.8.

VFA solution #

31
Solution occurs in 2 other media (first defined in Medium J133).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acetic acid 17 ml - -
Propionic acid 6 ml - -
n-Butyric acid 4 ml - -
iso-Butyric acid 1 ml - -
n-Valeric acid 1 ml - -
iso-Valeric acid 1 ml - -
DL-alpha-Methyl butyric acid 1 ml - -

Salt solution No. 1 #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 7.8 44.782
Distilled water 100.00 ml - -

Salt solution No. 2 #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.47 g 4.7 34.537
NaCl 1.18 g 11.8 201.916
(NH4)2SO4 1.20 g 12 90.813
CaCl2 0.12 g 1.2 10.813
MgSO4 x H2O 0.25 g 2.5 20.77
Distilled water 100.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 17.192 51.116
Trypticase peptone 1.91 -
Glucose 0.478 2.651
Cellobiose 0.478 1.395
Yeast extract 0.478 -
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