J144
144 .
MODIFIED NOS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Treponema denticola JCM 8226
This strain uses the standard medium without modifications.
Main sol. J144 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| 1 Combine the above ingredients and bring to a boil in a round bottom flask while flushing with O2-free N2-H2-CO2 (85:10:5) gas mixture. Stopper anaerobically, wire or seal closed and autoclave at 121C for 20 min. After autoclaving, allow to cool, open without admitting oxygen and while flushing with gas, add the following ingredients which have been filter-sterilized ahead of time: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Heart Infusion Broth
(BD-Difco) |
1.25 | g | 12.376 | - | |
|
Trypticase peptone
(BD-BBL) |
1.00 | g | 9.901 | - | |
|
Yeast extract
(BD-BBL) |
0.25 | g | 2.475 | - | |
| Sodium thioglycolate | 0.05 | g | 0.495 | 4.339 | |
| L-Cysteine HCl x H2O | 0.10 | g | 0.99 | - | |
| L-Asparagine | 0.25 | g | 2.475 | 18.735 | |
| Glucose | 0.20 | g | 1.98 | 10.991 | |
|
Resazurin
(0.1%) |
0.10 | ml | - | - | |
| Distilled water | 94.00 | ml | - | - | |
|
NaHCO3
(7.5%) |
2.67 | ml | - | - | |
| TPP/VFA mixture | 2.00 | ml | - | - | |
|
Rabbit serum
(heat-inactivated at 56C for 1 hr) |
2.00 | ml | - | - | |
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Thiamine pyrophosphate
(0.2%) |
1.5 | ml | - | - | |
| VFA solution | 1.0 | ml | - | - | |
| Distilled water | 8.4 | ml | - | - | |
| 1 Mix together and filter-sterilize. Store at -20C. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Isobutyric acid | 0.5 | ml | - | - | |
| 2-Methylbutyric acid | 0.5 | ml | - | - | |
| Isovaleric acid | 0.5 | ml | - | - | |
| Valeric acid | 0.5 | ml | - | - | |
|
NaOH
(0.1 N) |
100.0 | ml | - | - | |
| 1 Add volatile fatty acids to about 70-80 ml of NaOH solution and mix. Add NaOH solution to bring final volume to 100 ml. If not used the same day, filter-sterilize and store at 4C. | |||||
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Treponema denticola JCM 8226
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Heart Infusion Broth | 12.376 | - |
| Trypticase peptone | 9.901 | - |
| L-Asparagine | 2.475 | 18.735 |
| Yeast extract | 2.475 | - |
| Glucose | 1.98 | 10.991 |
| Show more | ||
