18 .
LACTIC ACID BACTERIA BROTH
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Equipment needed:
Final pH:
5.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone
(BD-BBL) |
10 | g | ||
|
|
Yeast autolysate | 5 | g | ||
|
|
Sodium acetate | 12 | g | ||
|
|
Glucose | 10 | g | ||
|
|
Solution A | 5 | ml | ||
|
|
Solution A | 5 | ml | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 5.1-5.3. | |||||
Solution A #
200
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
K2HPO4 | 10.0 | g | ||
|
|
KH2PO4 | 10.0 | g | ||
|
|
Distilled water | 100.0 | ml | ||
| 1 Solution B: | |||||
|
|
MgSO4 x 7 H2O | 4.0 | g | ||
|
|
NaCl | 0.2 | g | ||
|
|
FeSO4 x 7 H2O | 0.2 | g | ||
|
|
MnSO4 x n H2O | 0.2 | g | ||
|
|
Distilled water | 100.0 | ml | ||