JCM 190 . LYS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 0.999 7.341
(NH4)2SO4 1.00 g 0.999 7.56
NaCl 0.20 g 0.2 3.419
MgCl2 x 6 H2O 0.20 g 0.2 0.983
CaCl2 x 2 H2O 0.05 g 0.05 0.34
Trace element solution SL8 1.00 ml - -
Sodium lactate 2.20 g 2.198 19.613
Yeast extract 0.50 g 0.5 -
Vitamin B12 5.00 �g - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.8.
Solution occurs in 6 other media (first defined in Medium J190).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 5.2 g 5.2 -
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 -
H3BO3 62.0 mg 0.062 1.003
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 17.0 mg 0.017 0.1
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium lactate 2.198 19.613
(NH4)2SO4 0.999 7.56
KH2PO4 0.999 7.341
Yeast extract 0.5 -
NaCl 0.2 3.419
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
phyla 1 3 strains
oxygen aerobe 2 strains
temperature mesophilic 3 strains