194 .
MMYS-II MEDIUM
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Equipment needed:
Autoclave
Final pH:
7.8
Select a strain (optional):
Solution I #
501
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 1.00 | g |
|
|
NaCl | 8.20 | g |
|
|
MgCl2 x 6 H2O | 0.20 | g |
|
|
CaCl2 x 2 H2O | 0.05 | g |
|
|
Trace element solution SL8 | 1.00 | ml |
|
|
Sodium DL-malate | 3.60 | g |
|
|
Yeast extract | 1.00 | g |
|
|
Na2S2O3 x 5 H2O | 0.50 | g |
|
|
Distilled water | 500.00 | ml |
Solution II #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Potassium phosphate buffer
(pH 7.8, 20 mM) |
500 | ml | ||
| 1 Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7-7.8. | |||||
Trace element solution SL8 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Na2-EDTA | 5.2 | g |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
ZnCl2 | 70.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
H3BO3 | 62.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
CuCl2 x 2 H2O | 17.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 1000.0 | ml |