J195
195 .
THERMOPROTEUS NEUTROPHILUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J195 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Brock's salt base solution | 1000.00 | ml | - | - | |
| NaHCO3 | 0.85 | g | 0.85 | 10.118 | |
|
Yeast extract
(BD-Difco) |
0.20 | g | 0.2 | - | |
| Sodium dithionite | 25.00 | mg | 0.025 | 0.144 | |
|
Sulfur
(powder) |
8.00 | g | 8 | 249.454 | |
| Resazurin | 0.40 | mg | 4.0e-4 | 0.002 | |
| 1 Mix ingredients, except yeast extract, sodium dithionite and sulfur, and adjust pH to 6.5. Autoclave, then cool under H2-CO2 (80:20) gas mixture. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Filter-sterilize NaHCO3 as an 8% solution while gassing with N2. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and NaHCO3 solutions, and sulfur to the medium under a H2-CO2 (80:20) atmosphere. Readjust pH to 6.5 by further gassing with H2-CO2 (80:20) gas mixture, if necessary. Prior to inoculation, add filter-sterilized 2.5% sodium dithionite solution. Pressurize inoculated bottles to 100 kPa H2-CO2 (80:20). | |||||
Solution occurs in 13
other media (first defined in Medium J165).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 |
| FeCl3 x 6 H2O | 2.00 | mg | 0.002 | 0.007 |
| MnCl2 x 4 H2O | 1.80 | mg | 0.002 | 0.009 |
| Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - |
| ZnSO4 x 7 H2O | 0.22 | mg | 2.2e-4 | 7.7e-4 |
| CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 |
| Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 |
| VOSO4 x n H2O | 0.03 | mg | 3.0e-5 | - |
| CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 6.5 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 8 | 249.454 |
| (NH4)2SO4 | 1.3 | 9.838 |
| NaHCO3 | 0.85 | 10.118 |
| KH2PO4 | 0.28 | 2.058 |
| MgSO4 x 7 H2O | 0.25 | 1.014 |
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