21 .
LACTOBACILLUS MEDIUM III
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Equipment needed:
Final pH:
5.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone , tryptic digest | 10.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
5.0 | g | ||
|
|
Beef extract
(BD-Difco) |
2.0 | g | ||
|
|
Glucose | 20.0 | g | ||
|
|
KH2PO4 | 0.5 | g | ||
|
|
K2HPO4 | 0.5 | g | ||
|
|
FeSO4 x 7 H2O | 10.0 | mg | ||
|
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
|
MnSO4 x n H2O | 7.5 | mg | ||
|
|
Tween 80 | 1.0 | ml | ||
|
|
Ethanol | 40.0 | ml | ||
|
|
Sodium acetate | 20.0 | g | ||
|
|
DL-mevalonic acid | 30.0 | mg | ||
|
|
Agar | 15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 5.2. | |||||