J267
267 .
AN1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J267 #
ml
Equipment needed: Anaerobic workbench
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD-BBL) |
10.0 | g | 10 | - | |
| K2HPO4 | 1.5 | g | 1.5 | 8.612 | |
| NaCl | 2.5 | g | 2.5 | 42.779 | |
| Sodium thioglycolate | 1.0 | g | 1 | 8.764 | |
|
Sulfur
(powder) |
8.0 | g | 8 | 249.454 | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix ingredients, except sodium thioglycolate and sulfur, and adjust pH to 7.3. Autoclave the medium under a N2 atmosphere. Separately autoclave sodium thioglycolate as 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Under a N2 stream, mix the medium and sulfur in culture vessels and seal with butyl rubber stoppers. Prior to inoculation, add the thioglycolate solution to the medium anaerobically. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase peptone | 10 | - |
| Sulfur | 8 | 249.454 |
| NaCl | 2.5 | 42.779 |
| K2HPO4 | 1.5 | 8.612 |
| Sodium thioglycolate | 1 | 8.764 |
| Show more | ||
