298 .
HALOSIMPLEX MEDIUM
What is this page?
Equipment needed:
Final pH:
3.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glycerol | 2.5 | g | ||
|
|
Sodium pyruvate | 2.5 | g | ||
|
|
K2HPO4 | 0.5 | g | ||
|
|
(NH4)2SO4 | 1.0 | g | ||
|
|
KCl | 4.0 | g | ||
|
|
NaCl | 200.0 | g | ||
|
|
MgSO4 x 7 H2O | 20.0 | g | ||
|
|
Trace metal solution | 2.0 | ml | ||
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.15. | |||||
| 2 For solid agar slopes or plates, add 20.0 g Bacto agar (BD-Difco). | |||||
Trace metal solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuSO4 x 5 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 100.00 | ml | ||
| 1 Adjust pH to 3.0 - 4.0 with HCl. | |||||