JCM 319 . JCM MEDIUM No. 319

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal salts solution 5.00 ml - -
Phosphate solution 10.00 ml - -
Vitamin solution 1.00 ml - -
(NH4)2SO4 0.50 g 0.492 3.724
Na2S2O3 x 5 H2O 2.48 g 2.441 9.835
Sodium succinate 2.00 g 1.969 12.148
Yeast extract
(BD-Difco)
2.00 g 1.969 -
Casamino acids
(BD-Difco)
2.00 g 1.969 -
Distilled water 1000.00 ml - -
1 Adjust pH to 7.2.
Solution occurs in 3 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na3-EDTA 4.12 g 4.079 -
FeSO4 x H2O 1.11 g 1.099 6.468
MgSO4 x 7 H2O 24.65 g 24.406 99.02
CaCl2 x 2 H2O 2.94 g 2.911 19.8
NaCl 23.40 g 23.168 396.446
Trace elements solution 10.00 ml - -
Distilled water 1000.00 ml - -
Solution occurs in 4 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 75 g 75 551.126
K2HPO4 78 g 78 447.823
Distilled water 1000 ml - -
Solution occurs in 3 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 100 mg 1 8.123
Thiamine HCl 100 mg 1 -
Biotin 5 mg 0.05 0.205
p-Aminobenzoic acid 50 mg 0.5 3.646
Vitamin B12 1 mg 0.01 0.007
Calcium pantothenate 50 mg 0.5 1.049
Pyridoxine hydrochloride 50 mg 0.5 -
Folic acid 50 mg 0.5 1.133
Na3-EDTA 200 mg 2 -
Distilled water 100 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2.441 9.835
Sodium succinate 1.969 12.148
Casamino acids 1.969 -
Yeast extract 1.969 -
K2HPO4 0.768 4.408
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