333 .
POTATO-SUCROSE AGAR
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Equipment needed:
Autoclave
Final pH:
5.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Potato | 200 | g | ||
|
|
Sucrose | 20 | g | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 5.6. | |||||
| 2 Wash potatoes with tap water, peel and cut into 1 cm cubes. Rinse with tap water quickly and boil 200 g of potato cubes with 1.0 L of distilled water for 20 min. Mash and squeeze through a muslin bag. Add agar and boil until melting. Add sucrose and mix thoroughly. Make up to 1.0 L, adjust pH and autoclave for sterilization. | |||||