JCM 342 . BRAIN HEART INFUSION AGAR WITH SOIL EXTRACT

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Equipment needed: Autoclave

Final pH: n.d.

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ml
Compound Amount Unit
Brain heart infusion broth
(BD-Difco)
37 g
Soil extract 1000 ml
Agar 15 g

Soil extract #

1 Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation.