JCM 346 . R2A AGAR

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Equipment needed:

Final pH: 7.2

Select a strain (optional):

ml
Compound Amount Unit
Yeast extract
(BD-Difco)
0.50 g
Proteose peptone no. 3
(BD-Difco)
0.50 g
Casamino acids
(BD-Difco)
0.50 g
Glucose 0.50 g
Starch
(soluble)
0.50 g
Sodium pyruvate 0.30 g
K2HPO4 0.30 g
MgSO4 x 7 H2O 0.05 g
Agar 15.00 g
Distilled water 1000.00 ml
1 Adjust pH to 7.2.
2 Comment: The premixed powder is available from Becton Dickinson & Co. as Difco R2A Agar.